Is it possible to use phosphate buffer instead of Tris HCl?

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How to do pca analysis of c-alpha atom of the protein?

i m interested in pca analysis of c-alpha atoms in gromacs for that i used the following gmx_mpi covar -s mdca.tpr -f mdca.xtc -o eigenvalca.xvg -v eigenvecca.trr -av average.pdb -n index.ndx but...

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How to do calculation of Louvain methods calculation for excel sheet and and is there any software available?

The Louvain method – named after the University of Louvain where Blondel et al. developed the algorithm – finds communities by optimizing modularity locally for every node’s neighborhood, then...

23 July 2024 6,659 0 View

Getting this error : gmx_mpi': corrupted size vs. prev_size: 0x0000000001d1b320 ***?

i am using the trjconv command in gromacs gmx_mpi -debug trjconv -s md.gro -f md.xtc -o utr.xtc -fit rot+trans and after few steps i got the error gmx_mpi': corrupted size vs. prev_size:...

17 July 2024 6,216 1 View

What is information diffusion in the social network?How a message got viral in social network?

Please give answer. Also explain mathematical equations behind this.

22 June 2024 6,869 2 View

Is there any drugs or natural ingrdients with similar mechanism like that of low dose Amisulpride?

Is there any approved drugs or natural ingredients with similar mechanism like that of low dose Amisulpride. Is there any specific dose or borderline dose reported at which amisulpride is both...

02 June 2024 5,586 0 View

Is there any way to separate sodium sulfate with water without adding heat to the solution?

20 May 2024 3,239 1 View

What is the physical significance of critical exponents( Beta, Gamma, and Dealta) at the Curie transition region?

06 May 2024 5,236 0 View

How to get dataset in social network analysis for decision making?

Decision is an important concept in social network. If any one have website link , please give link.

03 May 2024 4,367 1 View

WARNING: FENE bond too long: 1 7 8 32968539.141547877 (src/MOLECULE/bond_fene.cpp:83) ERROR on proc 0: Bad FENE bond (src/MOLECULE/bond_fene.cpp:85)?

# Polymer chain simulation with FENE and LJ potential in NVT ensemble # Initialization units lj # Use LJ units atom_style bond # Atoms and bonds boundary p p...

16 April 2024 5,828 0 View

Bond broken in fene potential?

i simulating a single chain polymer with 10 particle with fene and lj but if i increase the no of particle my bonds are being broken . im using parameters as below 20 50000.0 0.1 1000.0 1.0...

15 April 2024 2,748 0 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

What can be done when electronic minimization does not converge during geometry optimization in CASTEP?

Hello All, I've been facing difficulty in executing geometry optimization of a sodium polyanionic phosphate (Na4Fe3P4O15) crystal in CASTEP (please find the input and output files attached). When...

07 July 2024 8,626 2 View

Can I directly ligate Illumina adaptor to the end of ssDNA?

I have a sequence of ssDNA that I would like to directly ligate an Illumina sequencing adaptor to. As long as I have done the end repair (remove phosphates), can I directly ligate the adaptor to...

26 June 2024 4,522 0 View

Initial and final setting times of a calcium phosphate cement?

I'm measuring the setting time of a calcium phosphate cement using an apparatus that records how much resistance a needle (connected to a load cell) encounters over time. This process provides...

24 June 2024 7,534 5 View

Ni-NTA protein purification - how to obtain higher yield concentration?

Hello, I am trying to purify a 6X-His tagged protein using Ni-NTA chromatography. However, I obtain very less yield & concentration; and considerable protein in load flow through. Any...

16 June 2024 5,876 5 View

How to interpret this sentence making a HPLC mobile phase?

I'm following a paper for their HPLC methodology, but I'm having a hard time understanding how to make mobile phase A. To quote: "Mobile phase A is a mixture containing 0.2% potassium dihydrogen...

13 June 2024 8,321 1 View

I noticed some white growth in the phosphate buffer that was prepared just a week ago. What could be the cause of this, and how can it be resolved?

The buffer wasn't filtered.. Could this be the cause? Can we autoclave phosphate buffer to resolve this issue?

10 June 2024 3,970 2 View

Is a 5` phosphate needed for homologous recombination?

Is a 5` phosphate needed for homologous recombination? I am a bit confused whether phosphorylated DNA is needed to achieve efficient homologous recombination in e coli. I believe there are methods...

06 June 2024 7,065 1 View

How to prepare 100ml solution of 2.5% Glutraldehyde in 0.1M PBS(phosphate buffer) pH about 7, from 25% Glutradehyde and 1X PBS?

final solution volume is 100ml

03 June 2024 417 0 View

Can methyl cellulose be used as a binder in the preparation of zeolite pellets?

I am currently preparing zeolite pellets for adsorption applications, specifically for removing phosphate and ammonium from aqueous solutions. So, I need to know about the binder used during this.

27 May 2024 5,802 0 View

Tejas Sharma

Agree with Sayed Omar. Most of the buffers are specific for the type of tissue. As in case for brain tissue homogenization we use mostly phosphate buffer saline while tris-HCL is for heart. Even buffer criteria will change according to the procedure we want to execute, means what we want to analyze. Therefore, it is advisable to stick to protocol, unless and otherwise one want to develop a new method.

Manoj Suva

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