I am thinking to investigate the roles of a specific gene during the embryonic development stages in a crab species. I was just wondering to ask about the possibility of gene knock-down or over-expression before hatching in aquatic animals.
Dose any have some experience regarding the drug/chemical/RNAi delivery into eggs in any aquatic animals?
either use a morpholino RNA or try CRISPR - see our recent article about CRISPR in ascidians
Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation. DOI: 10.1007/978-1-0716-0974-3_13.
One problem you may face is that you do not have a genome or transcriptome. In that case you would need to sequence the part of the genome so that you can design the morpholino or CRISPR in order to perform knockdown (morpholino) or knockout (CRISPR). Unfortunately with CRISPR even if you do that you will not know what other sites in the genome the sgRNA has targetted - so maybe try both morpholino and CRISPR showing that both give the same phenotype.
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