welll, i am trying to dope DNA with organic phosphorescent dye. The UV-vis spectrum for samples where doping was performed showed red shifts in peaks as compared to DNA peak seen at 260 nm . while in PL, DNA with dyes showed blue shifts as compared to previous PL results for the same samples.
1.) The uv-vis spectrum for DNA+dye molecule in same environment. i observed the peaks obtained for DNA+dye are red shifted from DNA peak. so is this to be seen in PL spectrum in anyway.
2.) Now PL study was done twice for the same DNA + dye sample of whose UV-Vis spectrum was obtained . During the second attempt for PL, i found that the peaks were blue shifted when compared to previous PL spectra.
so my question is UV-vis spectrum is red shifted while pl is blue shifted, is there any relation between them to be so?