As per titled, is it possible? To my knowledge, carotenoid can be red or yellow. I am attempting to calculate carotenoid content of fish pink skin with value from colorimeter. If it is possible, what is the formula to convert colorimeter value to carotenoid content for fish skin with what assumptions are made? Appreciate if anyone have related journal to share. Thank you.
In La*b* color space a* is related to red-green and b* related to yellow-blue, thus you can maybe perform a combination of both these parameters sqrt(a^2 + b^2). Do you have any value to share ?
You have to remove a piece of skin, weigh it, homogenize, extract carotenoids and perform HPLC separation. Many substances interfere with colorimetric determination. It is possible to do a crude determination of carotenoids in carrot or tomato extract, where one carotenoid prevails in high concentration.
Estimating carotenoid from colourimeter readings? Carotenoid absorbs in a UV/Visible at 450 nm depending on the solvent and absorption coefficient. Carotenoid is quantified spectrophotometrically mostly by OCC and HPLC methods after fulfilling all analytical conditions. I suggest you stick to that instead of colourimetric estimation.
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