Triterpenoids mimic the structure similar to cholesterol and hence they may interact with it in the plasma. They are also difficult to separate using HPTLC.
Most triterpenes do not have strong chromophores for detection by the universal UV spectral detectos in HPLC.Usually the end absoption is used to detect them by HPLC-UV employing ~205-210 nm. I believe the best way would be to use HPLC-DAD- ESI MS/MS in sequence.
HPTLC is a semi-quantitative technique. There are a number of examples of triterpenes separations successfully done using HPTLC. One of the best examples is the separation of ginsenosides of Panax ginseng. You could download the CAMAG application notes that provides well separated constituents. This technique also provides for on-plate derivatisation which is quite handy. The only problem is that this technique is semi-quantitative (and linear in a short range of concentrations).
Hope these comments are useful
Sir, thank you very much for your kind and valuable suggestions. I would also like to know the availability of lupeol in plasma too. Kindly help[.
Sir, thank you very much for your kind and valuable suggestions. I would also like to know the bioavailability of lupeol in plasma too. Kindly help.
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