I have the results from an experiment . The groups are
1. Saline
2. Treatment
3. Saline + X inhibitor
4. Treatment + X inhibitor
In previous experiments, treatment had a remarkable effect. My hypothesis was that treatment acted by influencing X and inhibiting X will make Saline and treatment group same. The experiment seems to have proved my hypothesis.
My comparison groups are 1 vs 2 or 3 vs 4. I am not at all concerned about whether the mean of 3 or 4 are same as that of 1 or 2.
Results are shown below
To compare 1 vs 2 and 3 vs 4 I used two Welch’s t tests instead of ANOVA. Is the Welchs test robust in my cases (my data is normal but may not same variance)
Thanks