I have the results from an experiment . The groups are

1. Saline

2. Treatment

3. Saline + X inhibitor

4. Treatment + X inhibitor

In previous experiments, treatment had a remarkable effect. My hypothesis was that treatment acted by influencing X and inhibiting X will make Saline and treatment group same. The experiment seems to have proved my hypothesis.

My comparison groups are 1 vs 2 or 3 vs 4. I am not at all concerned about whether the mean of 3 or 4 are same as that of 1 or 2.

Results are shown below

To compare 1 vs 2 and 3 vs 4 I used two Welch’s t tests instead of ANOVA. Is the Welchs test robust in my cases (my data is normal but may not same variance)

Thanks

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