I am currently working on tomato fruit quality, doing QTL mapping and fine mapping of genes involved in volatile pathways. The volatile quantification is generally performed by our collaborator, using a GC-FID instrument. Now I am considering to implement the quantification in my department, but using HS-SPME-GC-MS. I am mainly interested in relative values, for each experiment I will be comparing two groups (two genotypes) and I need to be confident enough about whether they are different or not. Do you think that SPME and GC-MS are accurate enough to do so? Thanks in advance.
Yes. You can also try to confirm the results using standard addition method.
Regards
GB
You probably don't even need to use SPME. Just remember to keep the samples cool before analysis since VOCs are volatile! HS is primarily used to reduce sample matrix interferences.
Hi.
No problem. It should be pretty straightforward. The DVB/CAR/PDMS fibre should do it.
Cheers!
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