To perform nucleic acid quantification DNA or RNA, fluorescence measurements can be done with instruments such as fluorometers or microplate readers. I've got two questions to try and get my head around it:
- is fluorescernce measurement commonly used for DNA/RNA quantification?
- What's some of the main research topics where RNA quantification is carried out?
Please look at the following below links which may help you in your analysis:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106716/pdf/am003238.pdf
https://www.thermofisher.com/sa/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-quantitation.html
Thanks
Mr. Papon,
Please consider the discussion shown below. As is underlined, therein, optical spectroscopic methods do not provide reliable quantitative data, due to a set of reasons. The quantitative analysis of macromolecules can be carried out reliably only by mass spectrometry.
There is important to focus attention on: (i) Method performances of Fs and MS instrumentation; as well as on the (ii) chemometrics of the data processing methods of spectroscopic and spectrometric outcomes.
Discussion: https://www.researchgate.net/post/Can_I_use_digested_seed_sample_sample_was_digested_in_HNO3_H2O2_for_determination_of_total_protein_content_through_UV_vis_spectrophotometer
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