hello,
i am working on psychophillic organic solvent tolerant lipase, i have some struggle in purification part. if any one is working in this area please tell me.
There is a vast literature here (400,00 plus papers). See on ResearchGate S5 Lipase an organic solvent tolerant enzyme. For an organic solvent and surfactant-tolerant, thermostable lipase, see link. For lipases in organic synthesis in general see Kazlauskas.
https://www.cbs.umn.edu/explore/departments/bmbb/contacts/romas-j-kazlauskas/publications
Article S5 Lipase: An Organic Solvent Tolerant Enzyme
The research group where I did my PhD a few years ago, is currently working on cold-adapted lipases. You may look up Enzyme and Microbial Research Technology group from Universiti Putra Malaysia. They have collaborative research with Prof Romas Kazlauskas, a collaborator of this research group. He used to advise me on my research projects and expert on lipases and hydrolases.
thank you Roswanira A. B. Wahab.
the information u provided is very helpful for me. currently we are working on several aspects of cold adopted microorganisms, among one is my PhD topic. thank you so much for the information.
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