Interesting project!

More Philipp von Gillhaussen's questions See All

Why is my protein eluting in the salt wash when applying IMAC?

Hi everyone! I currently have the issue that my proteins are all eluting at the salt wash that I apply during my IMAC (HisTrap 5ml HP column). My proteins are all His-SUMO-tagged. They are...

25 May 2024 8,144 3 View

How do I prevent dark blue spots/smear and the running front of my BN-PAGE?

Hi everyone, we are trying to establish BN-gels in our lab and we are currently facing some problems. We would like to investigate protein-protein interaction of membrane bound proteins from cell...

18 April 2024 3,219 0 View

Which pH buffer for dye adsorption experiments onto activated carbon?

1.) We are conducting adsorption experiments with methylen blue onto powdered acitvated carbon (liquid batch). 2.) To ensure constant pH conditions we have worked with potassium phosphate buffer...

14 November 2023 1,232 3 View

What is the best way to measure the Economic and/or Damage Threshold for the use of Biological Control Agents in a specific crop?

I am currently working in the monitoring of pests of a group of crops. The main issue we're facing at this time is how to properly calculate the levels of each pest for which the application of a...

01 August 2023 4,689 1 View

Why would borate buffer change its pH upon dilution?

The Pierce Borate buffer sold by Thermo Fisher: 20X Borate Buffer is ideal for preparing sodium borate buffer solutions for use in protein modification procedures requiring amine-free buffer at an...

01 August 2023 7,541 4 View

How do regions of injection locking of three coupled nonlinear oscillators look like in a devil's staircase in 3D (or 2D plot of the rotation number)?

By a 3D devil's staircase I mean the plot of the rotation number (or any invariant property distinguishing states of the system) as a function of the ratios w2:w1 and w3:w1, where w1, w2 and w3...

26 June 2023 357 0 View

Can I change the effect size in SPSS from a logarithmic scale to a non-logarithmic scale?

Hey there, I‘m using SPSS for the statistical synthesis of my Meta-Analysis and I can only choose log(OR), log(RR) etc as an Effect sizes. Therefore my forest plot is also on a logarithmic scale…...

19 June 2023 8,478 2 View

How to condunct a survival meta-analysis when the data for standard deviation ist missing?

Hey there, I‘m currently working on a surivival meta-analysis and I use SPSS for my statistics. The problem is that I have a lot of data regarding mean OS / PFS, but no belonging standard...

01 June 2023 6,470 4 View

What is minimal time of hybridization for FISH?

Hello! I am curious about the minimum hybridization time for the custom locus-specific probes for FISH. I know that it should take at least 12 hours at 37 degrees Celsius, but is there an even...

31 May 2023 9,886 2 View

Chuangjian Li good morning, I am trying to establish an email link regarding your work on diffractions?

My email is [email protected]

21 February 2023 7,227 3 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Which test should be used to study association among demographic profile and awarness level?

i have to study the awareness and adoption level of cloud computing in a district of India. i also want to use association among demographic variables like gender, age, education, income etc and...

02 August 2024 2,420 3 View

What should I do in this situation ?

I'm an undergraduate university student conducting research on the effects of internet use on depression and anxiety among undergraduate Ghanaian university students: Mediation role of Internet...

31 July 2024 2,979 3 View

What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?

31 July 2024 3,533 2 View

In teaching with Evidence-Based Practices for students with ASD, what are some that can benefit from the use of AI?

I am a student in a master's program for Special Education and I am curious to see what Evidence-Based Practices that are used in teaching individuals with Autism Spectrum Disorder benefit from...

30 July 2024 5,611 1 View

Tips for teaching Orton Gillingham+ to students with autism?

I am beginning to use OG+ in my district and noticed during training there are a lot of materials and routines, which may be overwhelming for my students, especially those on the autism spectrum....

30 July 2024 5,695 0 View

How to use Desmond in HPC ?

Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...

28 July 2024 6,553 1 View

Como e a rotina na educaçao infantil ?

Compreender o pertencimento das crianças na educação através da rotina e acolhida

23 July 2024 486 0 View

What sustainable management strategies can be used to prevent soil erosion and role of biodiversity in sustainable agriculture?

22 July 2024 5,672 4 View