ImageJ can be used for this purpose. please search for it on youtube.
As Mohammed M.Obeid said you can use ImageJ software (free in net). you may need to find a calibration between pexiles and nm or micrometer. You should find the particle size for most particles by using the tools of the software.
@ Mohammed M. Obeid
You made my day, I was laughing aloud. " search for it [imageJ] on youtube"!
thank you.
Happy to make you laugh Dr. Vladimir Dusevich. I am not very good in English because I used it as second language and I am still learning. To correct that phrase, " search for the same topic on youtube". I hope the phrase is good enough to be understood by native speakers.
Dr. Vladimir Dusevich, you may a great scientist but we are on our initial stage. Everything is not available on books and if available, it may not be as interesting as we find in videos because in videos, the things get visualize. Also please don't mind, researchers like you are not much interested in teaching rather in criticizing. Also, there's no doubt that you don't have much time. I don't find that this is a matter of laugh or fun. I'm happy that atleast Dr. Mohammed M. Obeid tried to help me instead of criticizing anyone.
It is the first time when I disagree with Dr. Dusevich :-)
There are a lot of self-evident tutorial materials about ImageJ on Youtube, e.g.:
https://www.youtube.com/watch?v=JQAZ--c9YfI
So, I owe an apology.
Dear Mohammed M. Obeid, I apologise, I was wrong.
Dear Samiksha,
Please use ImageJ software to measure the particle size distribution. Follow the steps as I have mentioned below:
1. Just open the TEM/SEM image in ImageJ and calibrate it with the given scale.
2. Select the area in which you have to find particle size distribution and make its duplicate (ctrl+shift+D).
3. ImageJ---- Process----- FFT----- Bandpass filter ; and filtered the image..
4. ImageJ---- Adjust----- Threshold >>>> click on dark background, choose Over/under in coloumn 2 then adjust threshold by a bar and then apply. After this checkout dark background, choose B&W in coloumn-2 and apply.
5. Click on Analyze ----- Analyze particles
6. Now in Analyze Particles window click on Show—Outlines; check on Exclude on edges, and clear results ---- OK
7. If everything is OK, again click on Analyze particle, Choose size limit 2-infinity and Circularity 0.2-1 ( whatever you want).--- Display Result ---- OK
8. You have the final result. Save it in xlsx file. Open it in MS Excel and for each area find r and d values.
9. Copy all d values and paste in Origin at Y column, Select whole B column and plot statistics--- Histogram.
You have the final partical size distribution.
You can do it manually by measuring diameter of particle one by one and then draw in Excel sheet.
Regards
Harshit
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