Why do I keep getting smudged and wavy bands in certain lanes of my western blots?

Hi everyone, Been doing western blots for years now, recently however, whenever I run 2 gels, one of the gels would have a few lanes that exhibit wavy or smudgy bands at around 50 kDa (see...

16 November 2020 3,759 11 View

How to use Clamping and interpret its outcomes in MAXENT?

For my Masters project I have been creating SDMs using MAXENT, and the next stage involves using future climate projections in the 'projection layers directory' to see potential future...

25 June 2020 2,658 6 View

Data and modeling for vapor-liquid metastable states, spinodals?

What publications are available with actual measured data on vapor-liquid metastable states and spinodals and the modeling thereof?

16 February 2020 9,000 1 View

I seem to have Protein stuck on my C8 column. How do I clean it properly?

Hi everyone, I've been trying to detect a Peptide Hormone (ca. 9 kDa) by RP-HPLC using H2O + 0,1% TFA and MeOH + 0,1% TFA as mobile phases with a flow rate of 1 mL/min, a C8 column with a...

07 April 2019 3,053 11 View

In Black hole theory, have mathematicians finally lost all connection with physics?

18 November 2018 4,730 38 View

Model Implied Instrumental Variables experiences?

MIIV (http://fgme2017.de/kenneth-bollen/) looks like a very promising approach. Are there any experiences from research practice? Application-oriented publications? When is it appropriate to use...

09 February 2018 174 1 View

Which is the best culture media for culturing macrophages?

08 October 2014 3,945 19 View

Is anyone familiar with In-vitro RNA transcription for long >6 kb transcripts?

20 September 2014 2,928 2 View

What kind of statistical test should I choose if I want to test regression to the mean on related data set?

I conducted an experiment to investigate the effect of a physical therapy treatment on the signal intensities of the 5 lumbar discs (L1-2 to L5-S1) on MR images. I found that the signal intensity...

07 March 2014 4,762 6 View

Has the speed of light been accurately measured in space, and would it be the same as on earth?

I believe there is reason to expect that the measure of the speed of light on the International Space Station would be greater than it is measured at the surface of the earth. My expectation is...

10 November 2013 2,652 32 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

What does Servant Leadership mean today and in the future? What are the advantages and disadvantages?

In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...

02 March 2021 8,149 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

How to perform Haldane correction on SPSS?

I was wondering if anyone knew how to perform the Haldane correction for SPSS (this is the definition of the correction -...

21 February 2021 2,882 1 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

Are there papers on?

I'm looking for a papers concerning either Christianity or neopagan religions and social media. What role does social media play in the spread of these religious movements? Any suggestions on the...

15 February 2021 655 4 View

How to Measure Consumer Willingness To Change from petroleum-fueled vehicle to electric vehicle?

I wanted to calculate the probability of one consumer shifting from using a petroleum-fueled vehicle to an electric vehicle. Since my background is in engineering I do not know much about how to...

15 February 2021 9,190 5 View