Could you please describe the experiment in more detail?
You can use generic terms like I am studying arthropods on plants, or I am studying the effects of two drugs on bacterial growth and each drug is tested at five dosages. The more detail you provide the better RG will be at providing useful answers. I have no problem suggesting that you kill another 10,000 bacteria, but another 10,000 mice would be a different issue.
I think you should explain your study in more detail. Instead of simply specifying how many variables you have, try to start with the research question you are interested in. For example, are you trying to find some differences between these two samples?