The sequencing is performed by many companies. only full genome sequencing without closing gaps may cost approximately 2000-3000 Euros. The time taking work is gap closing, annotations, comparison, synteny, evolutionary, and other functional analysis. The complete packages according to minimum publishable requiremnts will be around 8000-10000 Euros, or may go upto 14000 Euros. I think prices will be almost same in any part of the world, except a minor cost difference,

Thank you sir .what about the plasmid alone.

Plasmid sequencing is very easy. You can do it by yourself. You need the sequence of just one region of it and then design primers. you can use primer walking to cover the sequences. If you dont know the sequence of your plasmid (complete new), then you can digest it with any restriction enzyme (choose an enzyme by screening several). after digestion just ligate a small known DNA fragment to it, and design primers to this known fragment and perform primer walking. You can do it in your lab easily.

Hope it will work for you.

You can get the sequencing done at MTCC, NCCS.The cost of partial sequencing is around 3000 Rs. while for complete sequencing it is around 10,000/- you can get the information through their website.Besides there are few national research labs which would do your work free of cost for partial sequence as a part of their metagenomic work.If it is a fungal culture get it done at Agarkar research Institute.

I told you the bacterial sequencing cost, which i paid to one of the company last week for sequencing of our Bacterial strain (specific serotype). Complete sequence with bioinfo analysis cost 10, 000 Euros and it was the cheapest service in whole europe. companies such as MWG, ATGC, BGI and many others (according to the quotations we got). Usually one read for a plasmid (500-1000 bp) will cost 500-600 indian rupees. I never heard any service below it. Ketna, Can you please send me some Journal articles where Indian Scientists published full genome and performed genome sequencing at such a cheap cost? I may also ask for such a cheap sequencing from same company/Institute!

Regards,

At Ome Research Facility, Dept of Animal Biotechnology, Anand Agriculture University, Anand, Gujarat we extend the facility on actual cost basis. We use two sequencing platforms -GS-FLX (Roche) and PGM Ion Torrent (Invitrogen). Please J. Bacteriology for our genome announcements.

http://mtcc.imtech.res.in/fee.php(16s & 18s rDNA)

http://www.nccs.res.in/dnaseq.html(16s rDNA)

http://www.aripune.org/Services.asp(18s rDNA)

Dear Ketna,

I know Prof. Souche at NCCS, since last 10 years. They are using sequencing usually for characterization of spp. Full genome sequencing is not to sequence only 16S and 18S rDNA. Its much more. I am agree that sequencing step is the only easy one, but then gap closing and annotation are tedious and time consuming works. So bigger the genome of an organism, more the time you have to consume. Then each gene should be matched with databases like KEGG, TIGR, Uniprot, etc, to annotate the function. it is performed at individual gene level. Then you have to analyse how much intergenic regions and regulatory elements, evolutionary and synteny analysis, comparison with other known genomes of similar spp. The sequencing services that are available in those institutes (as you told) only covers your small requirements. like single or few gene sequencing, cloning vectors sequencing etc. I am sure non of these facilities, you mentioned, will promise to do genome sequencing in 10000 indian rupees.

Regards

Dear Ketna,

Now I have seen your links, becuase it was not opening in my station. Its only one gene sequence, for 10000 indian rupees. You can do it in 1000-2000 indian rupees, if you have a PCR machine and 16S, 18S primers. after doing PCR send it to to any sequencing company then it will read it is 500-600 indian ruppes per sequence.

Regards,

You can do it at kemri-cdc

http://www.seqsondemand.de

Contact de la société :

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DE-37085 GÖTTINGEN

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Téléphone:

Fax:+49 (0) 551 370 0010

Tel: +49 (0) 551 370 0012

At Ome Research Facility, Dept of Animal Biotechnology, Anand Agriculture University, Anand, Gujarat we extend the facility on actual cost basis. We use two sequencing platforms -GS-FLX (Roche) and PGM Ion Torrent (Invitrogen). Please refer J. Bacteriology for our three genome announcements