Is it possible to provoke a measurable CD8 response by in vitro re-stimultaion, and if so which is the preferred method: ELIspot or intracellular cytometry?
Dear Juan, you have a lot of possibilities. As the most direct approach to functions and specificity of CD8 T cells, I would suggest detection of CTLs. You describe your problem too briefly. So, you had not any answers before.
definitely ICS or tetramer unless your frequency is lower or far lower than 0.1%. The only advantage of ELISpot is you could potentially detect lower frequncy as you could dump a million cells in a well - if there is little to no background. However, you have purify your input cell population and you can get contamination easily
You have several ways to do it, for instance: inoculated the peptide in the mouse, after collected the PBL and in cultur stimulates with the peptide stop in 18 hours with brefeldin collected and do flow ciytometry with one antibody against CD8.
Other way is directly from mouse inoculated and cytometry. More in my opinion the first way is more specified
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