I want to do MIC and MBC test on streptococcus mutans, i have muller Hinton ,and BHI medium, is there any way that i can do this with out using blood?
the problem is I cant find blood in my university.
Dear Dr. Ghiasi,
you must be used CLSI principle and you can find this principle in attached file page 56 and you can buy this culture from http://zistroyesh.com/ and contact via telegram @BioGrowth
Yes you can
@StefanZimmerman
Dear Dr.Zimmermann
thank you for your response.
there are also other bacteria that i have to test my reagent against that, so i think i have to use same media for test them all
best regard
@Farzin asghari sana
Dear Dr.Asghari
it was very helpful i am really thankful
sincerely
Yes BHI can be used for S.M
Yes, I'm working with S.Mutans using BHI to determine MIC n MBC without blood. Have a try and see the results for instance
THB culture medium works well for your MICs test.
MIC could be work with BHI agar with blood. I used horse blood.
Where as BHI to work with, there is no need the blood. I am working with Streptococcus Mutans
the peptide which i have tested is about 100aa(hepsidin+ hydrophobin) and i haven't seen any inhibition zone, can it difuse through the muller hinton agar?
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