What is are the different classifications of a tool changer?

I have roamed the internet trying to see the different types of tool changers available but I found no definitive answers, could you kindly help me?

02 February 2021 5,036 1 View

How to calculate covariance of Expectation Maximization Clustering?

I am working with MatLab R2017a, I have to implement EM clustering. But I am stuck with initialization of covariance in it. I have to calculate covariance using the dataset which is comprised of...

25 December 2020 4,620 3 View

MatLab code for Expected Maximization clustering of n-Dimensional data?

I need matlab code for expected maximization clustering of n-Dimensional data. I have searched and found code related to 1-D and 2-D. Can someone refer the code for n-D .

22 December 2020 5,110 3 View

Where can I find papers related to microstructure of inconel alloy 800?

I need to define the microstructure of inconel 800, but I cannot find any literature related to it. Literature is about incoloy 800 mostly which is a bit different. Can anyone tell me if there...

07 December 2020 3,137 3 View

Which software application is best for Anova analysis?

I want to perform "ANOVA" analysis of three different vegetation indices. anyone know how can i perform and which application /software are suitable for this. Thank you in advance.

16 November 2020 2,599 4 View

How can I specify the parameters for nano creep?

Which parameters should I employ for nano creep testing of Titanium and Inconel alloys? Should I load it with a constant load rate or strain rate? What should be the maximum load? What should be...

29 October 2020 5,727 4 View

How to find the elastic work using nanoindentation load depth curves?

I am using work of indentation approach to find out the values of hardness of Ti and Inconel alloys using nanoindentation load-depth curves. I can find the formula for total work. Whereas for...

13 October 2020 2,778 5 View

How to measure the volume of hydrogel?

08 October 2020 2,745 9 View

Why nickel oxide nanoparticles do not have single band gap?

band gap for nickel oxide nanoparticles is 3.85 to 3.45 eV

23 September 2020 260 4 View

How to Determine LD50 of a test compound by Lorke's Method if your mice (mus musculus) dies at the lowest concetration i.e. 10mg/kg?

I have used Lorke's method of determining LD50 of my test drug. The lowest dose according to this method is 10mg/kg and my mice died at this dose within the 30 minutes of IP injection. Does that...

22 September 2020 9,959 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View