According to Epoch website, these columns (Cat 21980-010) should work with Qiagen Maxi prep buffers. I tried 5-6 times and for several of those columns, QBT buffer doesn't even run through. It starts to drip and stops. Some are extremely slow and several of them worked just fine. If it works, amount of DNA and its quality were equivalent to Qiagen. I contacted the company but not too much help. They simply says it works on their hand. I prepared their columns as below and move to Qiagen protocol from addition of QBT buffer.

3. Shake the GenCatch Maxi Column vigorously then

equilibrate the column by applying 5ml of 98%-100%

ethanol. Allow the column to be empty by gravity flow

and discard the filtrate.

The company says this ethanol solution actually comes out of the column but it was simply soaked up in the column and never dripped. I am ready to throw out these columns since I don't feel like wasting Qiagen BBT buffer but I thought I would ask if anybody can give me advice.

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