Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

Langevin Dynamics in shear flow?

Hi, We are trying to simulate particles with Langevin dynamics in a shear flow by Lees-Edwards boundary conditions in MD simulations. It seems thermal forces and shear flow do not go hand in...

23 February 2021 1,761 1 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Which is the best software or programming language (user friendly) for analysing data?

I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...

20 February 2021 3,950 15 View

Hi..how to isolate crude HDL (all subfractions) from human serum by size exclusion chromatography?

I would like to know about isolation of crude HDL from serum by SEC. Is it possible to perform with a serum sample volume of

19 February 2021 5,576 2 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Do you have any experience with transfection of EBV immortalized B cells with Lipofectamine?

I know that efficiency of transfection for primary B cells is very low with Lipofectamine. But I did not try that for EBV immortalized B cells. Do you have any experience with transfection of EBV...

05 February 2021 4,159 1 View

Spss won't divide my independent variables into two levels when i run a two way anova test. can anyone explain why this is the case?

i have two IVs and four DVs, initially, i was going to run a two way manova test but my DVs are not correlated, so i was told it's better to do individual two way anova for each DV. however,...

16 February 2021 3,390 7 View

How to analyse multiple Likert scales?

Hello everyone! I've encountered a problem when trying to figure out the methodology of my dissertation. The study has multiple variables and will be, in general, rather complicated, but my only...

02 February 2021 2,086 3 View

Having the moderator continuous or dichotomous? Using MANOVA or the Process macro (Hayes)?

Hi community! Currently finalising my Master thesis research. I manipulated my independent variable to have three conditions. Originally, I proposed a 3(3 conditions of independent v)x2...

30 January 2021 7,821 6 View

Study Design for 3 Interval DVs, mixed IVs?

I'm conducting a study on mental health terminology on social media for a group project. We have three interval DVs measured through likert scales and three IVs. The IVs are social media usage...

28 January 2021 2,871 3 View

What analysis method for 2 DVs and 6 IVs?

I want to test if job type, personality type and gender of a person have an effect on how their effectiveness and hireability is perceived. So my independent variables are job A and job B,...

15 January 2021 8,809 3 View

Are normality checks required for large sample sizes?

07 January 2021 6,360 2 View

MANOVA with 1 independent (predictor) variable and multiple DVs: how do I interpret and report the results?

The aim of my thesis is to see whether anxiety score can predict sleep quality and dream content. So for this, there is one continuous variable that I want to use as a predictor on multiple...

27 December 2020 790 8 View

Is a multivariate linear mixed model appropriate to analyse differences over time?

Say I have a data set of 32 study participants. The dependent variables are two different physiological reactions to stimuli. These are repeated measures, recorded over 16 trials. There are two...

13 December 2020 7,218 3 View

How do you perform and interprate a MANOVA analysis with no fixed factors?

Hello, I am doing a manova analysis (i.e. 4 DVS), and I only have covariates as IVs because it's a correlational study. I'm having trouble finding resources on how to do this with SPSS and...

08 December 2020 7,760 10 View

Output of rankMANOVA in R?

Dear all, I am working on data including two responses (count data, relatively correlated~R2=0.4, non-normal distribution, and violated the homogeneity of covariance) and three independent...

26 November 2020 711 3 View