Contamination is rare on plates and morphological characteristics are also as usual Rhizobia but chemical test making me ambiguous. Can you help me by giving any helpful information.
Dear Ali
They may be fast growing and salt stress resistant Bradyrhizobium thats why they grow so fast.....
incubate your plates further upto 6-7 days, slow growers may appear. Upload picture of fast growers on Congo red medium
I am culturing on CRYEMA media and I have inoculated at least 20-25 times and subcultured only one isolate got that taken a week and did not grow on 2-3% NaCl. So, it is making me confused that why other isolates are showing different characteristics.
if you are sure that the fast growers are morphologically (colony on CRYEMA) like Bradyrhizobium , better to identify one or two of them by 16S rDNA sequence analysis
16s rRNA gene is a housekeeping gene present in all bacteria. The presence of 16s rRNA gene in an organism confirms that this organism is a bacterium. If I analyze it, then most probably I will get that it is a species of bacteria because the morphological features are more or less same of soil bacteria. But I am concerned about Agrobacterium, it is near about Rhizobia. Moreover, I have tested it by inoculating with new born soybean plant. It made nodules in sand but not in 1% agar medium. My another confusion is that my control replications also produced nodules though I have not input bacterial inoculum.
How I can confirm that it is either Bradyrhizobium or Agrobacterium, do you know about any selection medium that is specific for Rhizobia?
Dear Ali,
Have you tried Yeast Manitol Agar (YMA) medium to grow those strains? This medium is specific for rhizobia.
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