Standard IEC 6090 states duration of 1 min however industry practices have been cited at 1-2 seconds. Looking for an official reference that allows shorter period of duration.
I'm assembling one PCR fragment (4.2 kb) with my vector, pECFP (4.8 kb), and transforming them into NEB 5-alpha Competent E. coli cells. Both the fragment and the vector were purified using the...
04 March 2024 1,090 5 View
Hello. Does anyone have any experience and tips on producing quality lysotracker and mitotracker stains in neurons? I can get a signal for both, but they're quite inconsistent-- I often get a...
26 February 2023 2,737 1 View
Hello everyone, first I will like to get ahead of the jargon-confusion with all these abbreviations. Intrinsically conductive polymers (ICPs) also called conjugated polymers, doped polymers or...
07 September 2022 3,900 0 View
Hi, I was trying to extract DNA with TRIzol. When I added chloroform to my sample with TRIzol and after centrifugate it, my phases were inverted. (Photo below) -De antemano muchas gracias
29 August 2022 2,553 4 View
Hi there! I've been looking everywhere for a compatible humidifying pan for our Sanyo MCO-18AIC incubator to no avail. Does anyone have any suggestions for which pan might be compatible with this...
05 July 2022 2,497 0 View
How can calculate the energy efficiency of a hydrogen produced from agricultural residual biomass gasification process in Aspen plus software?
31 March 2022 4,113 3 View
Hello all! I am new to this community and I am working on my first research study for my dissertation. I am having a very difficult time finding participants. I just don't understand how people...
12 September 2021 6,025 17 View
My co-author and I are working on a paper using the Economic Freedom of the World and 19 countries (sample cannot be increased because it is focused on a specific Region). The number of years is...
18 August 2021 1,274 2 View
I am looking to help dilute this down to the above concentrations.
29 July 2021 2,695 2 View
formal IDS consultation, compared with informal or lack of IDS consultation, reduces risk for new bacteremia episodes and improves long-term prognosis up to 10 years. why?
01 June 2021 8,122 3 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...
07 August 2024 5,177 3 View
Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...
03 August 2024 7,843 1 View
What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?
29 July 2024 7,714 4 View
In some data sources it has been grouped in Q1 and some shows it is Q2.
29 July 2024 4,227 2 View
Dear Research Community I would like to invite Elctrical Engineering specialists to solve problems related to Ternary Algebra or tripple sets I have an algorithm that places in relation three...
28 July 2024 5,771 2 View
Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...
28 July 2024 6,515 1 View
Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?
27 July 2024 648 3 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
I am looking to purchase a photography kit for onsite metallurgical investigations, such as capturing photos of worn areas, hot tears, cold tears etc. Could anyone suggest me a good...
22 July 2024 3,490 2 View