I want to detect arsenate concentration in a sample of groundwater.
I will be modifying my HPLC instrument to be able to run an ion-exchange chromatography. However, the HPLC machine's detector is a UV-Vis. Since arsenate might not be detected through UV-Vis alone, I will need to either complex the arsenate so there is a chromophore, or I will need to modify my detector method.
Are there suggestions to how I will be able to detect the concentrations of arsenate through the UV-Vis detector?
You will have to put Variamine Blue as a reagent in your mobile phase (use the same MP as normal conductivity), since it is a direct method. See attached;
Data Spectrophotometric determination of arsenic in environmental...
Hi!
Probably a dumb question, but why are you going to do that? You can get better qualitative info and better LoQs without it..... I am genuinely curious.
Cheers!
Why not just use 'ion chromatography (IC is a form of HPLC)' to separate the As molecules and ICP to detect the As (SIM or selected ion mode, use the molecular weight). Arsenate will be a separate peak.
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