How/where to order magnetostrictive alloy for biosensor in low quantity (MOQ < 1kg)?

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Should GFP sequence be cut together with the IRES sequence?

Hello. I have a viral plasmid which contains a bicistronic region: CMVprmtr-GFP-IRES-PuroR. I would like to cut out the GFP sequence to get rid of the green fluorescence. In this case, would it be...

02 February 2021 1,644 7 View

Examples of SWAT used in water management in operational practice?

Do you know any examples when the hydrological model made using SWAT became some software product used in operational practice for water management (cases of the water reservoirs management are...

04 June 2020 4,191 1 View

What are these cells with highly condensed basophilic nuclei and a light cytoplasm between proximal tubule epithelial cells in the zebrafish kidneys?

Please help determine what cells are in the proximal tubules of zebrafish. They have highly condensed nuclei, but I suspect that this is not karyopiches of apoptotic cells, but mitosis of normal...

03 June 2020 4,093 5 View

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home?

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home (freeware)? Chromatograms were made by Waters UPLC I-Class PDA-MS. At the workplace,...

14 May 2020 5,383 3 View

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It is quite difficult to find these enzyme preparations at that moment. Can anyone tell me who I can contact on this issue with?

29 February 2020 2,130 3 View

How to dilute fish blood plasma?

We are encountered with a phenomenon when T3 FREE was estimated in fish plasma by ELISA (DRG tests): some samples are shown no significant differences in total and diluted plasma (in PBS pH7.4,...

29 September 2019 8,819 3 View

Does anybody know a database on fluorescent properties of chemical compounds?

Does anybody know a database on fluorescent properties of chemical compounds? There are many papers describing flurescent properties of various compounds. Is there any database on fluorescent...

06 August 2019 9,239 3 View

How shoud one switch off a rotary vane pump?

How shoud I switch off a rotary vane pump? Should I initially turn off the pump and then open the shutter to normalize the pressure? Or the order of the actions should be vice versa?

06 August 2019 9,982 3 View

Can anyone verify whether this is an artifact or a point mutation in these Sanger sequencing results?

Hello! I have really strange results in my sequencing chromatograms. The goal was to analyze a point mutation which is heterozygous (DNMT3A gene, in position R882). So I performed a Sanger...

17 May 2019 7,386 3 View

Good day! Can someone help me? How can the mixing speed be taken into account in the mathematical model of diffusion in a liquid medium?

19 March 2019 7,162 3 View

Which is the most efficient etching solution for nickel based alloys?

Which solution reveals the microstructure and features of nickel-based alloys?

02 March 2021 2,221 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Why CNN algorithm performs well in Network Intrusion Detection based on some recent resources?

Most of the papers have compared different hyperparameters such as layers, initializers, activation function, and optimizer algorithm in the CNN algorithm for detecting network intrusion. This is...

24 February 2021 4,578 4 View

How to determine solutionizing time for cast Al alloys?

For wrought alloys soaking time at solutionizing temperature is determined by thickness .Do we have such standards for cast Al alloys

23 February 2021 8,839 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Which thermal conductivity test recommended for Ag-Cu nanofluid?

I have prepared Ag-Cu alloy with polymer coating upon it.... how can I measure thermal conductivity of it ?

22 February 2021 5,915 3 View

How to generate AA6061 alloy or any aluminium alloy crystal in material studio?

I need to carry out molecular dynamics simulation on Aluminium alloy. For this i need the crystal structure that which could be further replicated to form a structure.

22 February 2021 1,410 1 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

22 February 2021 215 2 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View