you can spray Ag No3 (300 ppm) @ 3-4 leaf stage and 15 days after 1st spray he may get 10% of male flower in seedless cucumber, if its gynocecious line,or if its monoecious line you can directly self it.
"How farmer can produce the seed of seedless cucumber for multiplication and growing for next season?"
Farmers/growers buy seeds of seedless cucumber for next season. Most of these seeds are F1 hybrid seeds, and they produce hybrid seedless cucumber.
1. Nowadays, most cucumber cultivars (include seedless) grown by growers are hybrid cultivars. Hybrid cultivar has the benefit of 'hybrid vigor' and they produce marketable 'uniform' products.
2. Hybrid cucumber cultivars are produced from hybrid seeds. Like hybrid corn, growers buy those hybrid seeds produced from companies.
3. Companies develops inbred lines, and use 2 inbred lines to produce hybrid seeds for sale. Companies usually set up plots (some with nets) for inbred line seed production and hybrid seed production.
4. So, as long as those inbred lines are maintained, they can always be used to produce hybrid seeds, and sell to growers to grow hybrid cultivars (including hybrid seedless cultivar).
5. Farmers or growers cannot save seeds from hybrid plants for next season use.
1. If plant-tissue-culture (PTC) technology can produce progeny with exact characteristics like their F1 hybrid parents, that will be great. However, PTC can cause phenotypic change due to somaclonal variation.
2. Other concerns are:
(a) Cost of operation. Which operation is cheaper? PTC technology or conventional breeding?
(b) Legal problem. Companies producing these hybrid seeds can take one to court for infringing their IP (Intellectual Property), if one uses plant materials derived from their hybrid seeds for tissue culture. Unless, you produce your own F1 elite lines.
Companies can produce F1 hybrid seeds or tissue-cultured seedlings (from F1 hybrid plants) for sale. However, tissue culture can cause phenotypic variation due to somaclonal variation.
Researchers have compared the phenotypes and genotypes of plants derived either from tissue culture of F1 plants or F1 seeds (see attached paper). I think more studies are needed.