I could not understand why someone can ask this question?

Is it the lack of basic understanding?

Please read this

Book Microbiology of the biogas process

Mr. ABHIJEET, Thank you for your interesting answer, but as you know I'm free to ask any question I think is important

January 8, 2023

Dear Benchouk-Rahim,

If you are culturing your organism(s) in a sealed bioreactor, you can try replacing the air (and the O2) from the headspace by pumping nitrogen gas into the vessel. The N2 can be sterilized by pumping it through a sterile filter. Pumping can be maintained at a low flow rate for the duration of the fermentation. The slight positive N2 pressure will prevent O2 from entering the vessel. Don't overpressurize the fermentor; keeping the headspace to a minimum will also help.

I hope this information helps you.

Bill Colonna Iowa State University, Ames, Iowa, USA [email protected]

in a sealed bioreactor, the oxygen will be gradually consumed if the microbes you use can do both aerobic and anaerobic metabolism