A simple way, that I know, is use of Histamine chamber with Nebulizer.

In this, you have to fill the solution of your drug in nebulizer placed in one compartment of chamber, while in another compartment you have to put animal (Guinea pig, Rat. etc).

Switch on the nebulizer which will pump medicament with desired rate, which will be inhaled by animal.

Disadvantage: The precise dosing can't achieved!!!

Thanks a lot mr.tejas but the drug i want to admininter is oil soluble so can i use that oil also in histamine chamber ? Will it work ?

Hi there,

in my previous lab we have used syringe-based aerosol devices. They work quite well, especially if you can apply a short anesthesia.

Here is the link:

http://penncentury.com/products/liquid-aerosol-devices/

Good luck with your studies.

Peppe

thanks Mr. giuseppe for ur valuable suggestion.

If nasal route to target brain, we use this http://www.ncbi.nlm.nih.gov/pubmed/11440750 slowly administering small volumes to an anesthetized animal in a supine position. But this uses an aqueous sol'n.

To administer through Nebulizer you required your drug in Solution form.

For that just mix oil with suitable solvent Ex.: DMSO. That could solve your problem...

thanks Yelena mam.

thanks once again to mr. tejas. i will discuss with my guide about DMSO, because its a new thing for me.

Miss Yelena i have gone through that link but only abstract is available there. i was not able to get the method meterial and method of drug administration. will u please provide me perticular that part ?

Dear Dr. Tyagi

There is numerous literature available. Try these papers:

Development and evaluation of olanzapine-loaded PLGA nanoparticles

for nose-to-brain delivery: In vitro and in vivo studies. U. Seju, A. Kumar, K.K. Sawant. Acta Biomaterialia 7 (2011) 4169–4176

Formulation and Characterization of Nanoemulsion-Based

Drug Delivery System of Risperidone. M. Kumar, K. Pathak. A. Misra. Drug Development and Industrial Pharmacy, 35:387–395, 2009

respected Sumeet: thanks for the valuable suggestion, but second paper suggested by you is not in albino rats . it is in sheep and that also they have taken nasal mucosa out of the body so not helpfull for me and the first paper suggested by you is paid journal. any how thanks for suggestions and waiting for next usefull suggestions from ur side.

yours sencerely

dr.kartikey

Dear Dr. Tyagi,

I can forward the papers to u. Plz mail ur mail id

It will be a great favour . My id is [email protected]

I have mailed some articles

Thanks ! Mr sumeet, i got ur mail. After going through them i will inform you.

With regards.

Dr.kartikey