Hello,
you can test the effectiveness of disinfactants using standard microbiology tests such as those used in researching the antimicrobial activity of substances. For example the technique of wells on agar in Petri dishes, you can also find the minimum inhibiting concentration of your disinfectants using 96-well plates.
There is also the technique in which you add your disinfectant to a liquid bacterial culture and then you have to monitor the bacterial growth or its inhibition by measuring the OD at 600 nm.
The dilution test is used to determine the chemical disinfection effectiveness on an inanimate surface. in these test bacterial culture is required to be suspended in the disinfection solution.
Suspension/dilution is not the right method for surface disinfection. It offfers no surface and suspended cells are more easily killed than cells oin a surface.
Use dilution that offers cylinders for surface relevance is the most relevant test.https://microchemlab.com/test/aoac-use-dilution-test-aoac-95514-95515-96402/
There is a specific EN standard, based on the type of disinfectant, its use and application (skin, surfaces, etc.) and the type of microorganism on which you intend to check the antimicrobial effect, which you must perform the test according to its instructions.
for example:
DIN EN 1040 : Chemical disinfectants and antiseptics – Quantitative suspension test for the evaluation of basic bactericidal activity of chmical disinfectants and antiseptics – test method and requirements
DIN EN 1275:2006, Chemical disinfectants and antiseptics – Quantitative suspension test for the evaluation of basic fungicidal or basic yeasticidal activity of chemical disinfectants and antiseptics – Test method and requirements
EN 1276 standard specifies a suspension test for establishing whether a chemical disinfectant or antiseptic has bactericidal activity.
etc.
For better understanding, please see the link below:
Disinfectant (realworldlabs.co.nz)
I suggest a test according to the pharmacopoeia.
https://www.drugfuture.com/pharmacopoeia/usp35/PDF/0619-0622%20%5B1072%5D%20DISINFECTANTS%20AND%20ANTISEPTICS.pdf
Use serial dilution Disinfectants and disc diffusion method for antibiotic (Disinfectants) susceptibility testing.
I think you need to consider the MIC (minimal inhibition concentration) and the MLD ( minimal lethal dose) of the substance.
Suggested to look into my article here;
Classic and alternative disinfection practices for preventing of hospital-acquired infections: a systemic review
Article Classic and alternative disinfection practices for preventin...
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