We want to investigate petroleum ether and n-butanol extracts. We have detected fatty acids and terpenoids by GC-MS analysis in these extracts. Is it possible to use DMSO as a pre-solvent?
Glycolipidsare used for solubilization and for serological activity more than 90% of the total fatty acids. An improved antigen is needed for use in complement fixation tests in which antigens are used as polysaccharide esters.
Yes, you can use DMSO or Tween. Try to solubilize in concentration lower than 0.05%. They may have effects in high concentration?
Kind regards
Thank you for both answers! I found one article (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1456728/) where DMSO is used as a pre-solvent with final concentration 0,3% in the reaction mixtures. We will see the concentration effect in the study.
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