By the ISO 3496:2007 it takes 16hrs to digest the sample for determination of hydroxyproline. I was wondering is there any way to shorten the digestion in half or less.
Kindly check https://www.researchgate.net/publication/51777520_Rapid_determination_of_collagen_in_meat-based_foods_by_microwave_hydrolysis_of_proteins_and_HPAEC-PAD_analysis_of_4-hydroxyproline
Also check https://www.buchi.com/us-en/node/7754
https://static1.buchi.com/sites/default/files/AN_053_2010_Detmination_Hydroxyprolin_Meat_Photometric_Method.pdf
I used the method described by Neuman and Logan, it is quite fast once you have the soluble and insoluble collagen content ready to go
Patience Coleman could you please tell me in which paper is the method described?
Sure...here you go
Patience Coleman Thank you very much
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