Hello,
I am currently working on my Master thesis and wanted to know that if we are producing biohydrogen (I-Stage) and biomethane (II-Stage) in a two-stage process from microalgae simultaneously and if we wanted to recirculate the digestate back again, which actually would contain methanogens, how can we separate it so it won't hinder the hydrogen-producing bacteria in the first reactor.
Why would you want to separate methanogens?
Have you proof that they affect the first stage?
In a mixed population there is always a competition for substrates or nutrients in general. The winner depends on environment and residence time.
My experience is that at high C-source concentration methanogens are the losers. Moreover methanogens grow slower, they are removed buy short residence time.
In any case you can reduce methanogens actìivity, if really necessary, by thermal effects or some oxygen supply to recirculated stream.
You have to disable methanogen for biohydrogen production. so you can use the acidic and thermal pretreatment.
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