I have a reaction in which two Dde protection, which i want to remove in solution phase reaction. I have done the deprotection of this group in solid phase peptide synthesis and its very easy to remove by using of 2% hydrazine in DMF. What kind of the solvent should i use to perform this reaction and how to work the reaction further.
I would perform the Dde de-protection in solution exactly as you did (2% hydrazine in DMF) in the solid phase. This reaction is really efficient (I would say in 10 minutes at 500mM conc. is already finished). Since you are working in solution phase, I suppose that you would like to avoid to use DMF. In that case, depending on your sequence and the degree of protection of your residues, I would try with MeOH, DCM, or acetonitrile. Keep in mind that, since the deprotection mechanism involve a nuclephilic attack, if you have neighboring amino groups (for example, a free Lys which is close to your Dde-protected Lys) Dde might migrate.
Check this out --> 10.1111/j.1399-3011.1998.tb00630.x
Best regards
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