What are the steps in the regeneration of 'fresh' DEAE Sephacel, which comes in the swollen form, in 20% ethanol. And what is the significants of each chemicals in these steps, can anybody explain?
Here is the instruction manual for this resin.
https://cdn.cytivalifesciences.com/api/public/content/digi-14011-pdf
To prepare the resin for the first use, you have only to replace the ethanol with the starting buffer until equilibrium is reached.
Regeneration is to be done by washing with a strong NaCl solution (1 M or 2M), which should elute just about anything that is bound by an ionic interaction.
If the column needs to be cleaned of hydrophobic substances, wash it with 0.01 M NaOH, then re-equilibrate it with the binding buffer until the pH is back to where it should be.
- Badges
- Science topic