Dear Xie,

Unfortunately, not all hiPSC lines are characterized by 100% differentiation efficiency. In case of lower efficiency of the differentiation protocol, cells enrichemt should be performed. I suggest you use the reagent set for Immunomagnetic negative selection kit (STEMCELL Technologies) called EasySep™ Human PSC-Derived Cardiomyocyte Enrichment Kit.

Remember to add ROCK inhibitor to the culture medium in which the cells grow after selection.

Good luck!