Just start dabbling around with C elegans (lab is planning to use it as future research model). Currently doing life cycle experiment, but the little guys keep disappear. How to count their life cycle if they keep missing??
Any idea why?
Either I do not know how to count or my nematodes have develop invisible skills!
p/s: I am using 3cm dish with 10mL NGM in each plate. Kept in 20 celcius fridge, inverted and daily observation.
They could just be crawling away on to the plastic and desiccating. I find this happens a lot if the food patch is close to or touching the plastic of a well or dish.
Thank you Mr. Lobos and Mr Weicksel.
Your's insights in this matter is really helpful.
I did find worms on the wall and burrowing in the agar.
Regards,
Qamarul
C.elegans exists and moves within a water film on the solid substrate, so be sure the agar plates are sufficiently thick and moist.
If you use a big number of worms, lost one or two worm won't be matter as you calculate it as censored data during data analysis. If you don't wish to have a censored in your data, prevent the worm from leaving the surface of the plate by place a ring of palmitic acid (10 mg/ml in ethanol). They won't escape.
Fabian (or anyone else familiar with this technique) could you share your way of placing the palmitic acid ring on the plate? i'd like to try this but am not sure how to do this properly.
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