I want to prepare the three levels of PEG-6000, which are 5% (mild drought), 10% (moderate drought), and 20% (severe drought). Many people said that to make 5% of PEG-6000, we need to take 5g of PEG-6000, dissolve it in water, and make a volume of 100 ml. In the same way, 10g for 10% PEG and 20g for 20% PEG. I want to confirm if this calculation is correct. The second question is how to apply this solution in potted plants grown in vermiculite culture? Is there any specific dose (5ml or 10ml per pot)? Most of the papers do not mention the dose per plant. your answers to my question will be highly appreciated. Thanks
Those calculations are are correct. You will need to add varying volumes in greenhouse experiments and add the data to one big table in your PERMANOVA as a variable :)
Andrew Paul McKenzie Pegman, thanks for your answer. Can you tell me how much concentration I should add for each peg level (5, 10, 15%) in each pot? Each tray has two pots, and I will add 4.4-litre water and Hoagland solution to the tray. How much concentration should I add for PEG 5% in the solution in the tray, or I directly dissolve PEG in that water and add water in trays?
I'm conducting an experiment involving the Hogland solution, in which I aim to induce two levels of drought stress (mild and severe) using either PEG-6000 or PG-8000.
However, I'm having difficulty determining the amount of PEG required per container(capacity = 830 ml).
I would greatly appreciate any guidance on this matter.
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