Does anyone know how to prepare the folowing vitamins? They will be used in bacteria culture media.
Biotin, Folic acid, Pyridoxine HCL, Thiamine HCL, Nicotinic acid, Calcium Paentothionate and p-aminobenzoic acid.
I know some of them have low solubility in water and others not, but I didn´t find any protocol.
thanks in advance!
This protocol for minimal media has a recipe for a vitamin solution on page 5.
http://structuralbiology.uchc.edu/protocols/pdfs/nmr_sample_preparation.pdf
Essentially you make them up in MQ then filter sterilise them. They should be kept out of direct light too.
They are all water soluble at the concentrations you would need to make stock solutions - at 100-1000x working dilutions. As Rory mentioned, filter sterilize the stocks. Store in a freezer (dark). For a limited period, one can store stocks in a cold room or at room temp but for extended periods of time, freezer is safer.
Dear Döhmer,
As Rory and Tausif suggested, dilute stock solutions, filter sterilize the stocks and store them in a dark freezer.
Best regards,
Kazemi
Not sure if you need a stock solution for Vitamin B12 too. If so, you may need to prepare a stock solution for it alone, I mean, separated from other vitamins, due to its very very tiny amounts requirement in culture medium, and then follow as suggested by all others. Best of luck.
- Badges
- Science topic
- Similar topics
- Biological Science
- Bioscience
- Biotechnology