How to merge coloc correlation coefficient data (Mander's or Pearson) from multiple single images together and get statistics?
Using Imaris or other turnkey programs, I can export Mander's or Pearson coefficients from 2-color images, but then, how do I take these numbers that I get from multiple images, and statisticize (yes I just made up that word) / combine them, and then compare treatment to controls?
Thanks!
EDIT IN ANSWER TO LAFIFI:
I much appreciate your answer and the links and files you uploaded. I am familiar with some of the sources you brought.
None of them show what to do AFTER I get coefficient values. For example, the Write document, shows how to use one of the imagej plugins, and shows a screen shot of the results window, and then just says :" ...exported to spreadsheet programs for further analysis ".
I am using the Imaris coloc module for 3D images. I can export the
coefficient values for every image. But then what do I do?
THAT is my problem. Can I do a simple average of the coefficient values from many images of the same treatment and compare to the average of the control?
cheers.
Dear Avi Jacob,
I suggest you to see links and attached files in subject.
-Colocalization Analysis - ImageJ
https://imagej.net/Colocalization_Analysis
-Colocalization coefficient - Scientific Volume Imaging
https://svi.nl/ColocalizationTheory
-New Algorithm to Determine True Colocalization in Combination with ...
journals.plos.org/plosone/article?id=10.1371/journal.pone...
-WCIF_ImageJ - Colocalisation Analysis - UHN Research
wwwfacilities.uhnresearch.ca/wcif/imagej/colour_analysis.htm
Best regards
I much appreciate your answer and the links and files you uploaded. I am familiar with some of the sources you brought.
None of them show what to do AFTER I get coefficient values. For example, the Write document, shows how to use one of the imagej plugins, and shows a screen shot of the results window, and then just says :" ...exported to spreadsheet programs for further analysis ".
I am using the Imaris coloc module for 3D images. I can export the
coefficient values for every image. But then what do I do?
THAT is my problem. Can I do a simple average of the coefficient values from many images of the same treatment and compare to the average of the control?
cheers.
Actually it is a pretty practical problem I will not be a great help, however your proposal will be to make not a simple average but a pendent average for a group of mages whose characteristics are similar and then you can compare it...
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