Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Is this grounds for theophylline toxicity even though her plasma is within the therapeutic range? if it is, how is that possible?

A patient was given 300 mg Theophylline taken twice a day for the treatment of her bronchial asthma and has a positive clinical response. She has been compliant in taking her medication however,...

09 February 2021 3,489 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 5,988 2 View

How can we increase awareness about Covid 19 prevention in our city among the local population?

The general public must be made aware of the mode of transmission, presenting symptoms and the measures that can be undertaken to prevent the spread of infection. Few options- Media, Webinars...

03 January 2021 3,538 13 View

Are the receptor proteins on the surface of infected cells will increase or decrease the susceptibility of antivirals?

Are the receptor proteins on the surface of infected cells will increase or decrease the susceptibility of antivirals?

22 December 2020 3,242 4 View

How to convert Terapeutic Human Doses to Cell Experimentation Doses?

"Toxicological evaluations revealed that Cur is found to be pharmacologically safe, even up to 12 g per day, as reported by several animal studies and in phase-I clinical trials Similarly,...

07 December 2020 3,012 1 View

I messed up the DMSO and serial dilution standards in my MSD assay...How can I save my data?

MSD requires 8 standards that are serially diluted down in order to produce a standard curve. In the final row of my MSD Assay, I accidentally pipetted DMSO (same volume) into the last 4 wells of...

24 November 2020 3,376 2 View

Will TNF alpha receptor 1/2 (TNFR1 or TNFR2) be up- or down-regulated when endothelial cells are exposed to TNF alpha?

Excessive exposure to TNF alpha leads to inflammation, oxidative stress, and other pathological responses in cells like endothelial cells. When cells are treated with TNF alpha, will the receptors...

21 November 2020 4,039 3 View

How to increase presynaptic neurotransmitter release artificially?

I would like to pharmacologically increase presynaptic neurotransmitter release in mice CNS in vivo (through the extracellular micro-infusion of a compound). Which strategy would you recommand?...

02 November 2020 5,645 3 View