Dear Community,
I am currently working on a project envolving the data extraction of the spectral charateristics of plants under artifical light for quality optimisation in PFAL Systems.
Therefore I have to access the Chlorophyll Fluorescence Yield (ChlFY) without the possibility of PAM AC signal filtering.
My tool is a simple spectrometer, with which I can acess the Raw Readings, the absolute PPFD readings and the Calibrated relative Reflection from 400 to 880nm. The sample speed is 1,2Hz. So short pulse modulation (PAM) is not possible.
But the system is designed to work with artificial light sorces (white LEDs + RGB LED) only. Therefore light intensity and spectra can be adjusted.
How can I acess Information abouth The ChlFY with this setup? Should I modulate some low frequency AC Light intensity change and check the ChlF change? Or is there some Index like NDVI/PRI to acess ChlFY?
Or did I missunderstand the Concept or difference between ChlF and ChlFY?
Thank you for your Help!
Thank you Md Abdur Rahim for your suggestion!
The paper you shared is about the quantitive determination of chlorophylls. This is done by measuring the absorbtion in the blue and red areas. Also he is working with extracted Chlorophyll, not on the Leaf/Canopy scale.
Unfortunatly this is not what I am searching for. I asked for a practical instruction or methode for the assesment of Chlorophyll Fluoresence Yield. In the presense of Intrinsic Light overlapping the Wavebands from 680nm to 800nm. My goal is to unbundle Chlorophyll Flourescence and Reflectance. Maybe you have another source for my particular question.
Thank you very much!
Have a look at Zarco-Tejada et al. (2018) where Ch-Fluorescence was measured using a 'FluorPen FP100' (http://www.psi.cz/products/pocket-sized-instruments/fluorpen) at Leaf/Canopy scale; and then used for inverse modelling purposes (using FluorFLIGHT 3-D radiative transfer model).
Zarco-Tejada, P. J., Camino, C., Beck, P. S. A., Calderon, R., Hornero, A., Hernández-Clemente, R., ... & Gonzalez-Dugo, V. (2018). Previsual symptoms of Xylella fastidiosa infection revealed in spectral plant-trait alterations. Nature Plants, 4(7), 432.
The paper you shared is about the quantitive determination of chlorophylls. This is done by measuring the absorbtion in the blue and red areas. Also he is working with extracted Chlorophyll, not on the Leaf/Canopy scale.
The paper you shared is about the quantitive determination of chlorophylls. This is done by measuring the absorbtion in the blue and red areas. Also he is working with extracted Chlorophyll, not on the Leaf/Canopy scale.
Chlorophyll fluorescence is measured by a fluorimeter
See my article:
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Paulownia tomentosa (Thunb.) Growth performance, photosynthetic status and bioaccumulation of heavy metals
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