Semen gradient centrifugation and sperm motility.
Hi,
For producing density gradients for separation of cells and
subcellular components by centrifugation or by sedimentation at unit gravity.
Neutral, highly-branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/ml can be achieved.
Better osmotic properties than sucrose. Preserves functional and morphological integrity.
Useful for separating cells that are sensitive to centrifugation
and for separating cells of similar density but different size
(under conditions of sedimentation at unit gravity).
Does not penetrate biological membranes.
Serves as the raw material for preparation of Ficoll-Paque gradients.
Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.
If you need technical note donot be hesitate to contact me
Good luck
Thank you all for valuable answers
I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...
03 March 2021 2,297 3 View
Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...
03 March 2021 6,223 5 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
01 March 2021 2,027 2 View
As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...
23 February 2021 3,175 3 View
23 February 2021 4,014 3 View
I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...
22 February 2021 10,099 5 View
My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...
22 February 2021 2,965 4 View
I performed a Proton NMR of graphene powder prepared by electrochemical exfoliation technique. It shows some peaks. But I can't understand it.
21 February 2021 8,947 3 View
Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...
17 February 2021 1,709 3 View
Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...
15 February 2021 6,749 2 View