How to improve reactivity of -NH2 toward Carbonyl Imidazole (CI) activated PEG?

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How to check the intermolecular interactions between an ion and a molecule using Gaussian software?

Hi everyone, I wanna check the intermolecular interactions between an ion (ex. Ca 2+) and an organic molecule (ex. chitosan). I did the same project before to check the electrical potential...

29 March 2024 9,340 1 View

Why is the absorbance of the egg/bovine serum albumin denaturation assay taken at 660 nm?

Denaturation assessments on egg albumin and bovine serum albumin suggest a link between tissue protein denaturation in living organisms and the onset of inflammatory complications. In this assay,...

09 January 2024 8,773 2 View

How can I prepare 0.1 M PBS with Na2HPO4 and KH2PO4?

What is the procedure for preparing a 0.1 M phosphate-buffered saline (PBS) solution using Na2HPO4 and KH2PO4?

19 December 2023 4,488 0 View

How can I adopt alternative methods to dry the protein fraction following acetone precipitation, specifically without freeze drying?

Could you kindly provide insights into suitable techniques or protocols for achieving effective protein fraction drying following acetone precipitation, considering the limitations associated with...

14 December 2023 7,729 1 View

What is the best sampling method/s?

I am targeting one group of unknown size of population (who engaged in some deviant behaviour). My unit of analysis is all active PhD and Doctoral students in Malaysian Public and Private...

07 December 2023 7,718 4 View

How can I separately isolate the R-phycocyanin and R-phycoerythrin from the crude phycobiliprotein extract by salting out ((NH4)2SO4 precipitation)?

Currently, I am working on the extraction of R-phycocyanin (R-PC) and R-phycoerythrin (R-PE) from red seaweed, Gracilaria tenuistipitata. The powdered seaweed sample was suspended in PBS (pH 7.0)...

17 November 2023 1,850 1 View

Im runing FPLC (Fast Protein Liquid Chromatograph) my ferritin in ph7 and ph 2 , does anyone knows how can I make sure how much is the retention time?

I have used PEG as supposed it would be stable in ph 2 and ph 7 and by shifting their peak can understand ho much is the retention time, but have nt yet get any good results

24 October 2023 8,236 2 View

How to estimate OD through ImageJ software using sections stained with Luxol Fast Blue?

Does anybody here know how to use ImageJ to determine the OD sections of Luxol Fast Blue stained? I need aid. I wanna compare the spare myelin in each group quantitatively using dyed spinal cord...

19 January 2023 8,818 1 View

How to get the results in the addams model as a time series?

The inputs of the model have been entered and the output is a file with the gst extension, but it displays the concentrations for one day as a time series, not annually, it does not display the...

16 December 2022 4,068 3 View

Can researcher self-Develop the Items in Survey?

Assalamualaikum and Hai fellow experts, May i know, can we develop our own questions (items) in the survey as additional measures for our questionnaire? What should we do after we self developed...

07 December 2022 9,428 2 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View

Why there are no peaks at downfield NMR of Cobalt and Nickel complexes?

I synthesized some paramagnetic cobalt and nickel complexes (expected to be Co(II) and Ni(II)) using a bidentate ligand - N of pyridine ring (aromatic) and O of P=O (aliphatic), and I obtained...

03 July 2024 3,410 4 View

Methods of O-methylation of amino acids without interfering the amine groups?

Hello guys. I have faced the problem of being in need of carboxyl-methylated amino acids. Amino acids I need are not available in the market so I need to synthesize them by myself. So thus I'm...

16 June 2024 9,920 3 View

How to purify Schiff base product?

How to stabilise the Schiff base product and avoid it from reverse back to the aldehyde and primary amine.

09 June 2024 9,264 5 View

How to select aminolysis reaction over transamidation reaction bw an amine and an amide ester?

Hi. i have a molecule it has both amide and ester bond. When i want to get second amide bond with ester part of the molecule by an amine, but it attacks to the amide bond also. In which conditions...

01 June 2024 9,863 2 View

Please share the HNMR sample preparation procedure for isotopic labelling test to confirm source of N2 during ENRR to ammonia.?

During electrocatalytic nitrogen reduction to ammonia, we perform isotopic labelling test using HNMR to confirm source of ammonia.

29 May 2024 5,165 0 View

Why is my protein eluting in the salt wash when applying IMAC?

Hi everyone! I currently have the issue that my proteins are all eluting at the salt wash that I apply during my IMAC (HisTrap 5ml HP column). My proteins are all His-SUMO-tagged. They are...

25 May 2024 8,144 3 View

Is there anyone that can assist be with removing excess 1-Iodo-Octane from from my product. Beside high vacuum distillation and kugelrohr what else?

I perform al alkylation reaction on an imdazole compound where the alkyl reagent (1-Iodo-Octane) was in excess. After the reaction was completed, I then performed an extraction, followed by...

24 May 2024 8,042 2 View

Does anyone know how to Coat polystyrene particles with APTES ?

I want to have amine group attached on the polystyrene particle surface. APTES can be used to do that.

21 May 2024 8,092 0 View