What kind of tape can be used to fix the samples on an XRD machine?

I have a very thin sample, the thickness is around 1 mm. I want to do X-ray diffraction measurement on the thin side of the sample. What kind of tape can I use to fix the sample, and will not...

13 February 2021 4,153 3 View

How can i detect the phenol red in mixture solution?

Hi. I'm trying to detect the phenol red in the mixture solution by HPLC. As you know, It is using for ph indicator. The problem is.. After making phenol red solution in 50 ml conical tube, I...

13 December 2020 5,511 4 View

How to deal with fMRI data with long task block (120s)?

Respected researchers, Recently, I have got a bunch of fMRI data collected from an experiment using a block design. However, it has a very long task block (lasting for 120 seconds) and containing...

11 December 2020 3,609 3 View

What are the effects of longer exposure to siRNA (for KD)?

1) I knocked down a protein and collected cells 24 h and 72 h after transfection with Lipofectamine (to note, 5 h after transfection medium was replaced with medium with FBS). 2) KD efficiency...

08 December 2020 9,850 1 View

How to export the standard XRD spectra of titanium alloy under different X-ray wavelength?

Hello all, which software can be used to export the standard XRD spectra of titanium alloy under different X-ray wavelength? How to operate? Such as 0.11 angstrom or 0.6887 angstrom (These X-ray...

07 December 2020 6,433 2 View

Why is hard to produce antibodies that are specific to a protein?

Some antibodies reveal nonspecific bands when a western blot is performed. Why is hard to find a good antibody for a protein? Antibodies produced using as the immunogen a synthetic peptide or a...

03 December 2020 208 3 View

Why different antibodies for the same protein stain different places?

I tried two antibodies and one show stain in the cytoplasm and the other one in the nucleus. Why? (Blue - dapi; red - mitochondria; green - protein of interest) And why is hard to find a good...

24 November 2020 6,612 7 View

Could bone tissue be saved in 70% EtOH after decalcification before processing?

I got the mouse tibia and femur tissue harvested, fixed with formalin 48h and decalcified with 14% EDTA 72h before paraffin embedding. This protocol works well for both HE and IHC. Now, for the...

18 October 2020 5,318 1 View

How to prevent protein gelation?

Hi :D I have a question. We expressed a protein from E. coli and we got purified peptide. But when we concentrate it with centrifuge filtration (like amicon), It became like a gel. So when we...

05 October 2020 3,727 2 View

Why are G-quadruplexes a thing? Why not A, T, or C-quadruplex?

Guanine-rich DNA sequences can fold into four-stranded, noncanonical secondary structures called G-quadruplexes (G4s). G4s were initially considered a structural curiosity, but recent evidence...

27 September 2020 9,105 6 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

How to use measure capacitance change in COMSOL time dependent study ?

I want to measure change of capacitance due to relative displacement between two parallel plates in COMSOL. Distance between two plates changes with time. So I'm using a time dependent study but...

26 February 2021 8,567 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Missing Data in Pretest and Post test- Experimental Research design?

Hello all, I am analyzing some pretest and post-test data that were given to evaluate knowledge of targeted vocabulary before and after the intervention. Pretest and post-test are identical and...

23 February 2021 9,400 7 View