Actually I found in previous research papers that both Cr forms i.e. trivalent and hexavalent are interconvertible. So I want to know the exact amount of both the forms of Cr in soil sample in which I have supplied only hexavalent Cr. It will help me to understand the mechanism behind toxic behavior of both the forms.
Praveen Kumar Determine Cr(VI) by diphenylcarbazide (DPC) and then determine total Cr using ICP or AAS, if you have these instruments. DPC is specific to Cr (VI), while AAS/ICP measures total Cr (VI and III). subtract the difference, which gives Cr (III).
If you don't have access to these instrument, then try this method
Article Speciation and determination of chromium in waters
Can you please elaborate what is ICP here. Thank you very much for suggesting me wonderful solution for this problem
ICP is ICPAES (Inductively Coupled Plasma Atomic Emission Spectrometer). There are many ICPs in Indian Institutes.
Cr(VI) as Yunus suggested, can be analyzed using UV Visible spectrophotometer. Its a simple color based analysis.
Total Cr through AAS (for ppm level till 3ppm) or ICP( for ppb level).
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