How to extract Rn values (fluorescence ratio without baseline correction) after a qPCR, using ths 7500 SDS Software (v.1.2.3)?

03 March 2013 1 8K Report

After running qPCR plates, we would like to analyse them using the LinRegPCR software, that allows for efficiency calculation within each well. It requires fluorescence ratio before baseline correction for background fluorescence.

However, in the 7500 SDS software, we only found 'Export'>'delta Rn' to get the baseline corrected ratio. Such baseline correction is applied during analysis and can either be automatic or manual but can not be eliminated altogether.

Has anybody ever encountered (and hopefully solved) the same difficulty?

Karen Reece

I think you could export the component view to get the raw fluorescence values, but you would would have to do the normalization with the exported ROX values.

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