How to extract pyocyanin for HPLC estimation from Pseudomonas aeruginosa?

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Bruce Neagle

See the attached articles below;

Article Pseudomonas aeruginosa Pyocyanin and 1-hydroxyphenazine inhi...

Article High-performance liquid chromatography analysis of Pseudomon...

Minakshi Singh

thank you Bruce Neagle

Nasser Belboukhari

I advice you to read this paper

https://www.sciencedirect.com/science/article/pii/.../pdf?md5

can I use nutrtient broth instead of King's A medium for extraction of pyocyanin???

No idea! Look at the growth requirements for P. Aeruginosa (the species may require special nutrients for growth).