Good day, Felix Sahayaraj Arockiasamy! Hope you are doing well.

I suppose you may start looking through the material with the standard methodologies, close to the one, enlisted below:

"SPNCCs were prepared by acid hydrolysis of the cellulose obtained as described elsewhere (Sanyang et al., 2016). Acid hydrolysis was carried out using 60 wt% H2SO4 solution and kept stirring with mechanical stirrer 1200 rpm for 45 min at 45 °C. The time of hydrolysis in this study was fixed at 45 min, which was found to be the optimum time (Bondeson, Mathew, & Oksman, 2006a). The ratio of the obtained cellulose to liquor was 5:100 (wt%) (5 g cellulose: 100 g H2SO4 solution). The hydrolyzed cellulose samples were washed four times by centrifugation (6000 rpm, 20 min, and 10 °C) to eliminate the leftover sulphuric acid. The suspension was then dialyzed against distilled water until a constant pH was reached. Then, the resultant suspension was sonicated for 30 min. Lastly, the suspension was freeze-dried at −110 °C using ethylene gas and stored at cool place prior to sample analysis (Bondeson et al., 2006a; Sheltami et al., 2012)."

Link: Article Isolation and characterization of nanocrystalline cellulose ...

Feel free to mention me in your response in case of any extra questions.

Best of luck in your research!

Yours sincerely,

M. Sc. Vadym Chibrikov

Department of Microstructure and Mechanics of Biomaterials

Institute of Agrophysics, Polish Academy of Sciences

Hi,

These articles may help you:

https://www.sciencedirect.com/science/article/pii/S240584402032329X

https://www.sciencedirect.com/science/article/abs/pii/S0926669020309924