Hi,
I've conducted crystal violet biofilm assay, testing iodine and chlorhexidine based disinfectants against E. coli (NCTC 11560) and S. aureus (ATCC 29213). With the iodine disinfectants, I got an expected pattern, where less biofilm is formed at the highest concentration of disinfectant and more is formed at the lowest concentration. However, in the case of chlorhexidine containing disinfectants, the pattern appears to be in reverse, with relatively strong biofilm formation at the highest concentration of the disinfectant, and the lowest growth at the lowest concentration.
The picture attached shows a two-fold dilutions of two iodine disinfectants (columns labelled GG and LD) and two chlorhexidine disinfectants (columns CD and SC) tested against E. coli biofilm. Has anyone witnessed similar patterns before or can suggest any literature explaining it?
Kind regards,
Kamilla
Dear Kamilla Anochshenko,
Please check
https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1472-765X.2007.02249.x
Good Luck
Yes, it's mentioned in literature that chlorhexidine can enhance biofilm formation of some strains especially when used in low concentrations
Article Chlorhexidine Digluconate Effects on Planktonic Growth and B...
Hi Kamilla,
It is an interesting finding, my recommendation is to supplement this data with XTT assay (metabolic activity) and OD600 or 660nm to assess biofilm thickness. These additional experiments will confirm there is no bias in your observation.
XTT Article Crystal Violet and XTT Assays on Staphylococcus aureus Biofi...
Optical density Article In situ non-destructive measurement of biofilm thickness and...
Good luck!
I agree with Miriam's views.. These papers will certainly help in your experiment results and observations can be explained accordingly.
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