Applying vacuum is correct, but you do not need to freeze your seeds. Freezing is only needed to avoid a collapse of structure for soft matter while drying. Use the Antoine Eq. or a similar one to calculate the pressure needed for the highest acceptable temperature and stay below this pressure. This would be for pure water. For a low seed moisture content, you need a sorption isotherm to compensate for the binding forces between the surface and the water molecules..
Hi Harald, I did not say that Saddam has to freeze dry the seeds. I only said "apply vacuum". I mentioned "freeze drying" just as a reference on how the drying is carried out under vacuum. If you want to be precise, Saddam wrote "without applying heat". If you do not provide heat, even under vacuum, there will be no evaporation. You must supply heat (as is done in freeze drying) if you want to evaporate the water.