What is/are the suitable adsorbent/s for separation of catechins (flavonols) by column chromatography? Will the separated fraction of individual catechin be stable?
Although it is very difficult to isolate catechins from column chromatography, probably the attached article could be helpful for you.
alot of methods can be used to isolate catechins ,I agree with Ruchi note, also you can see the attached file may help .
Why not use Sephadex LH 20.
Dear Kula Kamal
Please find some relvent information in attachement, which help you most for designing your study protocol.
Thanks you all your valuable suggestions. we are trying the separation with both silica and sephadex LH 20.
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