What should my PVDF membrane look like post transfer during a Western Blot?

When I perform western blot experiments I often get inconsistent results post transfer. What I mean is that when I look at my PVDF membrane after the transfer step I can often see the molecular...

08 September 2018 4,762 4 View

Why does my plasmid run heavy on an Agarose gel?

To test if my double digest of the pTriex-3 vector is working I ran my 'uncut plasmid', 'double digested plasmid' and 'enzyme 1 cut plasmid' and 'enzyme 2 cut plasmid' on an agarose gel. Double...

02 March 2018 4,174 2 View

Have my BL21 cells gained antibiotic resistance?

My BL21 cells when grown on an Ampicillin agar plate, completely cover the plate in a lawn as fast as 5 hours. They do this regardless of whether I transform them or not. A plate treated with no...

01 February 2018 321 5 View

How did my insert vanish from my plasmid?

Short version: My plasmid contained insert but later after a second transformation into a second cell line the insert vanished and does not show up on PCR or sequencing Long version: I used...

01 February 2018 6,654 9 View

How can I delete a large DNA fragment from my gene of interest using PCR techniques?

08 September 2017 1,966 6 View

What is the detection limit for a species (in time) of absorbance spectroscopy?

How long must a species last for, for it be detectable through typical absorbance spectroscopy. Say I have an enzyme with a turnover number of 300. Each reaction would take only 3.3 milli seconds....

07 August 2016 1,074 4 View

Do cumulitive effects on specific activity indicate a concerted mechanism?

I am looking at an enzymes reaction mechanism. I have investigated 2 mutations. both mutations individually reduce the enzymes catalytic activity. The 2 mutations combined render any product...

03 April 2016 2,664 10 View

Can you relate the energy of enzymatic steps to Kcat?

31 December 2015 3,312 7 View

Can anyone help with problems with non linear elution pH gradients during Chromatofocusing?

I am using GE healthcare poly-buffer 96 and PBE 94. I use a 0.025 M ethanolamine start buffer pH 9.4 and an elution buffer of PB 96 pH 6.0. I take care to degas all my buffers, keep the...

06 July 2014 2,644 2 View

Can anyone help with multiple bands after DNA double digest?

I am doing an Nde1 and BamH1 double digest. The gel is as followed Pet-28a with no insert (undigested), pet-28a (digested),Thermoscientific zip ruler express 2, pet-11a with insert (undigested),...

06 July 2014 429 7 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Can a semi-conductor material have different absorption coefficient?

Does the absorption coefficient of the semiconductor changes with the change in the structure of the semiconductor? If you have a thin film and nanowire of the same semiconductor, will the...

23 February 2021 9,799 4 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

absorption= 1-mag(S(FP1:1,FP1:1)*S(FP1:1,FP1:1))-mag(S(FP2:1,FP1:1)*S(FP2:1,FP1:1)) is it correct for absorption ?

hi everyone I am simulating a unit cell structure in HFSS using Flouquet port having two modes for absorption I have used this formula:- absorption=...

17 February 2021 9,178 2 View

Unusually Busy Raman Background?

Hello, I am observing a nearly periodic background spectrum for a wide variety of SERS substrates. I see this response (attached below) for bare Au films, Cu films, and Au nanoparticles on...

15 February 2021 9,922 5 View